The powers of PD-L1
Previous studies have tried using immunotherapies for type 1 diabetes, aiming to curb the autoimmune attack on the body’s islet cells. These attempts have failed, in part because the therapies have not targeted diabetes specifically. Autologous bone-marrow transplant — infusing patients with their own blood stem cells to reboot their immune system — has helped some patients, but not all.
“Blood stem cells have immune-regulatory abilities, but it appears that in mice and humans with diabetes, these abilities are impaired,” says Fiorina, in Boston Children’s Division of Nephrology. “We found that in diabetes, blood stem cells are defective, promoting inflammation and possibly leading to the onset of disease.”
A team led by Fiorina and first author Moufida Ben Nasr, PhD, also of Boston Children’s, began by profiling the transcriptome of blood stem cells to find out what proteins the cells are making.
Using a gene expression microarray, they found that the network of genetic regulatory factors (microRNAs) controlling production of PD-L1 is altered in blood stem cells from diabetic mice and humans. This prevents the production of PD-L1, even early in the disease.
They further showed that PD-L1 has a potent anti-inflammatory effect in the context of type 1 diabetes.
PD-L1 is known as an immune “checkpoint” molecule. It binds to the PD-1 receptor (inhibitory programmed death 1) on the inflammatory T-cells that are activated to cause autoimmune reactions. This causes the T-cells to die or become anergic (or inactive).
When Fiorina, Ben Nasr and colleagues introduced a healthy gene for PD-L1 into the stem cells, using a harmless virus as the carrier, the treated cells reversed diabetes in the mice. Fiorina and colleagues also found they could achieve the same effect by treating the cells with a “cocktail” of three small molecules: interferon beta, interferon-gamma, and polyinosinic-polycytidylic acid.
“We think the resolution of PD-L1 deficiency may provide a novel therapeutic tool for the disease,” Ben Nasr says.
Further study will be needed to determine how long the effects of the cell therapy last and how often the treatment would need to be given. “The beauty of this approach is the virtual lack of any adverse effects since it would use the patients’ own cells,” says Fiorina.
In collaboration with scientists from Fate Therapeutics (San Diego, Calif.), Fiorina and colleagues are working to optimize the small-molecule “cocktail” used to modulate the blood stem cells. The team has completed a pre-investigational new drug (IND) meeting with the U.S. Food and Drug Administration to support the conduct of a clinical trial in type 1 diabetes.
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